APPLICATIONS

Q-Sense systems are mainly used for characterization of bio-interfaces. Samples range from peptides to proteins to cells.



proteins: adsorption/interaction



polymers: multilayer formation



lipids: biosensor templates

               Proteins  Lipids  Polymers  Cells/Bacteria  Surfactants

With QCM-D, two parameters - frequency and dissipation - are monitored simul-taneosuly, in real-time, as molecular layers form on the sensor surface. This is what a raw data plot could look like:

  1. Binding of a small globular molecule
    Moderate frequency response, Δf (mass change), but low dissipation, ΔD(structural change).
  2. Binding of a large elongated molecule
    Forms a softer and thicker layer which can be seen by higher Δf and much higher ΔD levels.
  3. Rinsing with buffer
    The elongated molecule is removed, frequency and dissipation reduce again.

For all applications, Q-Sense systems enable:

  • Mass measurements. Nanogram sensitivity, less than 1% of a protein monolayer is detectable. 
  • Structural properties. Simultaneously determine the orientation of molecules at the surface. 
  • Real time, in situ and label free. Study kinetics, pH dependence etc. using the native molecule. 
  • Flexible choice of substrate. Metals, polymers; any material that can be applied as a thin film can be used.